Next-Generation Sequencing (NGS), also known as high-throughput sequencing or massively parallel sequencing (MPS), is a revolutionary nucleic acid sequencing technology following the Sanger first-generation sequencing. Its core principle is sequencing while synthesizing (SBS), which enables the simultaneous parallel reading of millions to billions of nucleic acid molecules. It can produce massive amounts of genetic sequence data in a single run. Compared with the first-generation sequencing, it has the core advantages of high throughput, low cost, high sensitivity, and multi-sample parallelism.
Whole Genome Sequencing (WGS): It covers the entire genome and is applied to population evolutionary studies, rare‑disease research, and comprehensive screening of tumor‑related variants. Whole Exome Sequencing (WES): It captures only coding regions, delivers high cost‑effectiveness, and is suitable for genetic disease research and tumor driver‑gene screening. Targeted Capture Panel Sequencing: It enables customized gene‑panel design and high‑depth sequencing, serving as mainstream solutions for clinical tumor companion diagnostics and targeted pathogen detection. Amplicon Sequencing: Applied to 16S/18S/ITS microbial diversity analysis and screening for mutations within target gene fragments. mNGS (Metagenomic Next‑Generation Sequencing): It enables unbiased detection of all microbial nucleic acids in samples and is used for complicated infections as well as research on intestinal and environmental microbiota.
mRNA‑seq: Quantifies gene expression, identifies differentially expressed genes, and analyzes alternative splicing. Whole‑transcriptome sequencing: Enables integrated analysis of mRNA, lncRNA, miRNA and circRNA. Single‑cell RNA Sequencing (scRNA‑seq): Conducts cell clustering, detects cell‑marker genes and characterizes tissue heterogeneity.
WGBS (Whole Genome Bisulfite Sequencing), ChIP-seq (Chromatin Immunoprecipitation Sequencing), ATAC-seq (Assay for Transposase-Accessible Chromatin sequencing) for sequencing of DNA-protein interactions and chromatin open regions.
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