Product Usage

After adding chloroform and homogenizing, the mixture was centrifuged and divided into three layers, including the upper colorless aqueous phase, the middle phase, and the lower red organic phase. The RNA was dissolved in the aqueous phase, and after removing the aqueous phase, the protein could be precipitated with isopropanol to obtain purified RNA; the soluble substances in the middle phase and the lower red organic phase could be precipitated with anhydrous ethanol to obtain purified DNA. There should be no protein contamination in theory.

The white flocculent substance in Buffer II is the SDS precipitated at low temperature. Before use, please place it in a water bath at 37℃ and shake well until the flocculent substance is completely dissolved.

This kit does not contain an endotoxin removal agent. The plasmids obtained through extraction can be directly used for cell transfection.

The recombinant protein of this product is tag-free.

Sure, both frozen (at -20℃ or -80℃), dried and fresh plant leaves can be used. However, when using fresh samples, the amplification yield is the highest.

PCR Enhancer can effectively enhance the amplification efficiency of fragments with a length of ≥ 4 kbp and high GC content (GC content > 70%). It can also effectively reduce the background of amplification products and inhibit the generation of non-specific bands. It is not necessary to add it in non-essential situations, as it may affect the stability of primer-template pairing.

A one-tube continuous closed-loop operation involves fewer pipetting steps, has an extremely low risk of cross-contamination, and is time-saving and labor-saving. It is highly suitable for clinical diagnosis and high-throughput large-scale sample screening. However, due to the shared buffer system for reverse transcription and qPCR, the reaction conditions often compromise between the two, thus making it impossible to separate the two-step reactions.

At present, this series of products have undergone agarose gel electrophoresis using semiconductor metal quantum dots. This can help the quantum dot samples settle in the sample wells, and has no significant effect on the fluorescence intensity. However, it should be noted that EDTA should not be included in the electrophoresis system at this time, as it will form chelation with the metal ligands of the semiconductor metal quantum dots, resulting in fluorescence quenching.

The seamless cloning kit can meet the seamless cloning needs in most scenarios. It can achieve seamless cloning for up to 4 fragments with a total length of 4000bp when dealing with multiple fragments.

If the number of used fragments is greater than 4, or the length of a single fragment is greater than 4000bp, it is recommended to use the enhanced seamless cloning kit.

The total number of fragments should be no more than 8 and the total length should be no more than 10,000 base pairs. There is no size limit for individual fragments.