Cat.No.：A02021A
Price: ￥180
Specifications：
Super high fidelity PCR premix (2×) contains DNA Polymerase, dNTPs and an optimized reaction buffer. When used, only template DNA, primers and deionized water need to be added to perform amplification. The DNA polymerase used is a new generation of ultra-high fidelity enzyme that has been modified by molecular enzymology. It has excellent amplification efficiency and wide template compatibility. This enzyme has 3′→5′ exonuclease correction activity, which can generate blunt-ended amplification products, and exhibits the following outstanding properties: extremely high fidelity (160 times that of Taq DNA Polymerase), fast amplification speed (up to 15 seconds per 1 kb), strong amplification capacity (can effectively amplify complex templates such as plant genomic DNA up to 15 kb), and at the same time, it has high sensitivity and good tolerance to amplification inhibitors. The unique stabilizers added to the premix can ensure that the amplification performance of the product remains stable even after undergoing up to 25 repeated freeze-thaw cycles.
Super high fidelity: Mismatch rate is lower than 4.4×10⁻⁷, which is approximately 2000 times higher than that of ordinary Taq enzyme (refer to the abm MegaFi Pro Fidelity system architecture);
Long fragment amplification capability: Can stably amplify target fragments of over 10 kb;
High specificity: Effectively inhibits low-temperature non-specific amplification;
Operational convenience: In 2× pre-mix form, reduces pipetting errors and improves batch-to-batch repeatability.
| Cat. No. | Component | Specification | Quantity |
| B02021A | Super HiFi PCR Master Mix（2×） | 1 mL | 1 |
| B02021A | Super HiFi PCR Master Mix（2×） | 1 mL | 5 |
Store at -20℃ and avoid repeated freezing and thawing.
Gene amplification, DNA fragment ligation, introduction of mutations, complete gene synthesis, DNA end repair, etc.
The amplification efficiency is low, and the bands are weak or no amplification occurs.
1. Reasons for template primers: Ensure the quality of the template and primers, and guarantee no degradation. Additionally, if the template contains a large amount of inhibitors (such as plant genomic template), it is recommended to use PCR enzymes with strong inhibitor resistance; one can also perform gradient dilution of the template for amplification and explore the appropriate template dosage. 2. Reasons for reaction system and conditions: Increasing the number of cycles, reducing the annealing temperature and humidity, and appropriately increasing the working concentration of Mg2+ can all improve amplification efficiency, but at the same time, it will lead to a decrease in specificity and fidelity. It is necessary to optimize appropriate conditions according to specific experimental requirements.
Low amplification specificity: primer dimers, background bands, tailing, etc.
1. Select heat-labile enzyme: Compared with ordinary PCR enzymes, heat-labile enzymes have higher amplification efficiency and specificity. 2. Optimize reaction system and conditions: Increasing the annealing temperature can effectively improve specificity. Additionally, reducing the number of cycles and appropriately lowering the working concentration of Mg2+ can also enhance specificity, but all of these will lead to a decrease in amplification efficiency. It is necessary to optimize appropriate conditions based on specific experimental requirements.
