Cat.No.：D20011A
Price: ￥
Specifications：
T4 DNA (T4 DNA Ligase) ligase is an ATP-dependent enzyme that catalyzes the formation of a phosphodiester bond between adjacent 5' phosphate termini and 3' hydroxyl termini in double-stranded DNA or RNA, connecting sticky-ended or blunt-ended DNA fragments. It can also repair single-strand gaps in double-stranded DNA, RNA, or DNA/RNA hybrids. It can be used for cloning PCR products, cloning DNA fragments generated by restriction endonucleases, and DNA and RNA connection repair, etc.
The product has a high purity and no residual endonuclease, exonuclease or RNAase activity, meeting the requirements of conventional ligation reactions.
| Cat. No. | Component | Specification | Quantity |
| B27011A | T4 DNA Ligase | 1000 U | 1 |
| B27012A | T4 DNA Ligation Buffer | 100 μL | 1 |
Store at -20℃ and transport at ≤0℃
This product can catalyze the formation of phosphodiester bonds between adjacent 5' phosphate termini and 3' hydroxyl termini on double-stranded DNA or RNA. It is used for connecting sticky-ended or blunt-ended DNA fragments, or for repairing single-strand cuts in double-stranded nucleic acids.
What are the differences between T4 ligase and the ligase of Escherichia coli?
The main difference between the two enzymes is that the E. coli DNA ligase cannot ligate blunt-ended dsDNA fragments. Both enzymes can be used to repair single-strand cuts in double-stranded DNA and perform sticky-end ligation. The E. coli DNA ligase is usually used for cut repair during the synthesis of the second strand of cDNA, as the T4 DNA ligase may cause the formation of chimeric insertions.
What are the common inhibitors of T4 ligase?
dATP, high concentrations (≥ 0.2 M) of salt ions, polyamines, spermidine and spermine, etc., can all inhibit the activity of T4 ligase.
Do both the vector and the inserted fragment need to be subjected to phosphorylation treatment?
Either the vector or the inserted fragment can be subjected to phosphorylation treatment.
