The thermal activation enzyme needs to activate its activity at high temperatures. It is recommended to set the pre-denaturation time to 3-5 minutes when using it. An excessively long pre-denaturation period will cause the enzyme to lose some of its activity, while a too short time will result in incomplete activation of the enzyme activity.
Thermal activation enzymes are mainly applicable to the following samples:
① Complex samples: For samples with many impurities such as tumors, blood, and soil, ordinary Taq enzymes are prone to causing false positives. Thermal activation enzymes can effectively resist interference from impurities, significantly reducing non-specific amplification and the formation of primer dimers.
② Low-abundance targets: Thermal activation enzymes can significantly improve the specificity and sensitivity of the reaction, allowing for more output of the target fragment. They are highly suitable for qPCR and low-copy gene detection.
