Other Tool Enzymes

The extremely heat-stable single-stranded binding protein (ET SSB) is a high-performance single-stranded DNA binding protein extracted from extreme thermophilic microorganisms. The protein exists in a monomeric form in solution and carries an exclusive OB-fold nucleic acid binding domain. It forms a polymer structure only when binding to single-stranded DNA.

This protein has sequence non-specific single-stranded DNA binding ability and can specifically bind to single-stranded DNA, without binding to double-stranded DNA or RNA. It can effectively protect single-stranded DNA from being degraded by nucleases, and inhibit the self-folding of DNA to form secondary structures, unwind the DNA helical structure, and assist DNA polymerase in efficient binding to the template, significantly improving the success rate of nucleic acid amplification and sequencing experiments.

The product has extreme heat resistance characteristics. Even after being incubated at 95℃ for 60 minutes, it can still maintain complete enzyme activity. The heat stability performance is far superior to that of the conventional Escherichia coli SSB protein, and it can directly withstand the entire PCR high-temperature denaturation steps without the need to add additional reagents midway.

1. Extremely resistant to high temperatures, suitable for high-temperature experiments.

The conventional Escherichia coli SSB protein is largely inactivated at 65°C, while this product remains fully active even after being subjected to long-term high-temperature treatment at 95°C. It can fully adapt to the PCR high-temperature cycling system without the need for additional supplementation. The experimental operation is more convenient.

2. Optimized template structure, significantly improving amplification effect.

It can stably protect single-stranded DNA templates, prevent degradation by nucleases and the formation of secondary structures such as hairpins and stem loops, effectively solving the problems of difficult amplification of complex templates, non-specific amplification, and low amplification yield.

3. Universal and non-preferential binding, wide adaptability.

It adopts a non-sequence-dependent binding mode, which can adapt to various different template sequences and is compatible with various mainstream molecular experimental systems such as PCR, qPCR, RT-PCR, and DNA sequencing.

4. Ultra-high purity, low interference from impurities.

After multiple purification processes, the protein purity is ≥ 95%, and the residual host DNA is extremely low. There is no contamination from exo-enzymes, endonucleases, RNase, etc. The experimental background is clean, and the data repeatability is extremely high.

5. Strong compatibility of buffer system.

It is compatible with most DNA polymerase reaction buffers on the market. It can be directly added to various nucleic acid reaction systems without adjusting the system ratio. It is ready-to-use and has full compatibility.

6. Exclusive optimization for multiple PCR and complex templates

For high GC, repetitive sequences, easily folded complex templates, and multiple PCR multi-primer systems, it can significantly improve amplification specificity and product yield, and reduce non-specific bands.

Instructions for use: This product is compatible with various polymerase buffer systems. General recommended dosage: add 200 ng ET SSB per 50 μL reaction system for optimal experimental results.

Buffer formulation: 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4 (25°C)

1. Long-term preservation: It is recommended to store at -25℃ to -15℃ in a sealed manner for freezing, ensuring the stability of protein activity;

2. Short-term preservation: For short-term use within one year, it can be stored at 4℃;

3. Storage restrictions: Do not repeatedly freeze and thaw. It is recommended to aliquot and store as needed after receiving the product; the freeze-dried powder version can be stored for a long time under a dry and sealed condition at 4℃.

1. Optimization of nucleic acid amplification: Significantly increase the amplification yield and specificity of common PCR, multiplex PCR, qPCR, and RT-PCR, and solve the problems of complex template amplification; 

2. DNA sequencing experiments: Stabilize single-stranded DNA templates, eliminate secondary structure interference, and improve the sequencing accuracy and read length quality of highly complex sequences; 

3. Protection of nucleic acid samples: Protect single-stranded DNA throughout the process, avoid nucleotidease degradation, and ensure the integrity of the template; 

4. Gene editing assistance experiments: Enhance the single-stranded DNA binding ability and chain replacement efficiency of RecA protein, and optimize homologous recombination-related experiments; 

5. High-throughput nucleic acid experiments: Adapt to various high-temperature, long-duration, and difficult molecular biology reaction systems, suitable for the development of high-throughput scientific research experiments.