Cat.No.：D11012A
Price: ￥100
Specifications：
High conversion rate: Conversion efficiency > 1x108 cfu/µg pUC19 DNA.
Stable genetics: Prepared through optimized process, it possesses stable genetic characteristics, ensuring the reliability and reproducibility of the experiments.
| Cat. No. | Component | Specification | Quantity |
| B15011A | Supercompetent Cell (E. coli, DH5α) | 100 μL | 10 |
| B15011A | Supercompetent Cell (E. coli, DH5α) | 100 μL | 20 |
Stored at -80℃ and transported with dry ice.
This product is applicable for gene cloning, blue-white screening, and plasmid stable amplification, etc.
What are the differences between DH5α and TOP10?
Both are common Escherichia coli cloning strains. TOP10 (genotype highly similar to DH10B) retains the mcrA locus, and thus may have a higher efficiency when transforming methylated DNA from eukaryotic sources (such as cDNA libraries). While the hsdR mutation in DH5α is more suitable for transforming non-methylated DNA obtained through PCR amplification. For the amplification of conventional plasmids, the functions of both can be basically interchangeable.
Can it be repeatedly frozen and thawed?
It is strongly not recommended to use after repeated freeze-thaw cycles. The transformation activity of competent cells stored at -80℃ or transported with dry ice can be reduced by more than 50% after one freeze-thaw cycle.
