Cat.No.：D22011A
Price: ￥
Specifications：
The site-directed mutagenesis kit is a reagent set that can introduce point mutations, insertions or deletions into any type of plasmid DNA. This product can quickly perform point mutations of plasmid DNA, mutations of multiple adjacent codons, and deletions or insertions of single or multiple adjacent codons.
This reagent kit provides DH5α glycerol bacteria, which can be used for the preparation of competent cells.
Low template usage: Exponential amplification
Simple operation: No need for special carriers, restriction sites or target plasmid methylation status, no need for separate steps to destroy the initial template
Widely applicable: Amplification length can reach up to 10kb
| Cat. No. | Component | Specification | Quantity |
| B29011A | Super HiFi PCR Master Mix（2×） | 125 μL | 1 |
| B29021A | Dpn I | 10 μL | 1 |
| B29031A | DH5α Competent Glycerol Stock | 200 μL | 1 |
| B29041A | Recombinase | 50 μL | 1 |
Store at -20℃ and transport at ≤0℃.
This product can introduce point mutations, insertions or deletions into any type of plasmid DNA, thereby achieving site-directed mutagenesis of plasmid DNA.
Does this kit support multiple point mutations?
It can be applied to multiple point mutations, but the success rate decreases when the number of mutation sites is greater than or equal to 4.
Do you support mutations of 50bp or longer in length?
The mutation site depends on the primer design. However, for base changes of more than 50 bp, if the restriction enzyme cutting site is suitable, it is recommended to attempt seamless cloning.
What are the key points to consider when designing primers?
The total length of the primers is generally 25–45 bp. The homologous sequences on both sides of the mutation site each account for 15–20 bp to ensure annealing specificity. Additionally, the mutated base should be designed as close as possible to the middle region of the primer.
