Cat.No.：D02101A
Price: ￥
Specifications：
This product is a pre-mix solution specifically designed for direct PCR of animal tissues/cells. It uses an engineered DNA polymerase that is resistant to PCR inhibitors and a dedicated lysis buffer system. It can directly initiate PCR amplification using a small amount of raw samples such as mouse tails, ear tags, muscle, blood, and cell precipitates as templates, without the need for a pre-nucleic acid extraction step. This significantly simplifies the experimental process and shortens the detection cycle.
Direct expansion without extraction: Use the lysis buffer or crude extract as the template directly, eliminating the DNA purification step.
Inhibitor tolerance: Highly tolerant to common inhibitors such as heme, humic acid, and polysaccharides.
High sensitivity: Stable amplification can be achieved with a small amount of sample.
Culturing solution support: Provide optimized rapid lysis buffer.
| Cat. No. | Component | Specification | Quantity |
| B02111A | Animal Tissue Direct PCR Master Mix (2×) | 1 mL | 1 |
| B02111A | Animal Tissue Direct PCR Master Mix (2×) | 1 mL | 5 |
Store at -20℃ and transport at ≤0℃
Rapid identification of experimental animal genotypes (for mice, rats, zebrafish, etc.), classification of livestock and poultry breeds, samples of animal organs / villi, screening of pathogenic agents of animal-origin diseases.
Is it better to use mouse tissue for direct PCR from the mouse's toe or tail? Is purification necessary?
No purification is required. Centrifuge to obtain the supernatant (optional). There is no comparison between the effects of mouse tails and mouse toes. Usually, mouse tails are used.
The positive control shows a specific band, while the sample being tested has no band or a weak band. Why is this?
The template amount was added improperly or the number of cycles was insufficient. It is recommended to optimize the template addition amount within the reaction system range of <5%, and appropriately increase the number of PCR cycles. It is recommended to use 35-40 cycles as the best option. Because the template is complex, generally, the PCR reaction needs to be 5-10 cycles more than using purified DNA templates.
