Cat.No.：D31011A
Price: ￥
Specifications：
pUC19 is a small, high-copy E. coli cloning vector carrying the ampicillin (Amp) resistance gene. Its lac operon region contains the CAP protein binding site, Plac promoter, lac repressor binding site and the 5'-portion of the lacZ gene. Insertion of foreign DNA into the multiple cloning site (MCS) within the lacZ gene inactivates the N-terminal fragment of β-galactosidase and abolishes α-complementation. Therefore, E. coli containing recombinant plasmids forms white colonies on the medium.
| Vector Type | Prokaryotic Cloning Vector |
| Cloning Method | Multiple Cloning Sites, Restriction Endonucleases |
| Vector Size | 2686 bp |
| Vector Tag | LacZ |
| Vector Resistance | Ampicillin (Amp) |
| Cat. No. | Component | Specification | Quantity |
| B62011A | pUC19 | 20 μg | 1 |
Which types of competent cells can pUC19 be used for?
pUC19 is suitable for most commercial competent cells, especially for strains used in blue-white screening, such as TOP10, DH5α, JM109, etc.
