Cat.No.：D11032A
Price: ￥100
Specifications：
Super competent cell TOP10 is an immediate-use chemical competent cell with the genotype F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697 galU galK rpsL (StrR) endA1 nupG. This strain lacks the recA1 and endA1 genes, eliminating the interference of recombinase and nucleases, enabling stable replication of high-copy-number plasmids. It is suitable for efficient cloning and plasmid proliferation. This product is prepared through a special process and uses optimized competent cell-specific storage solution to ensure the reliability and repeatability of the experiment. When using the pUC19 plasmid for thermal activation transformation, the transformation efficiency is > 1x108 cfu/µg.
High conversion rate: Conversion efficiency > 1x108 cfu/µg pUC19 DNA.
Stable genetics: Prepared through optimized process, it possesses stable genetic characteristics, ensuring the reliability and reproducibility of the experiments.
| Cat. No. | Component | Specification | Quantity |
| B15031A | Supercompetent Cell (E. coli, TOP 10) | 100 μL | 10 |
| B15031A | Supercompetent Cell (E. coli, TOP 10) | 100 μL | 20 |
Stored at -80℃ and transported with dry ice.
This product is applicable for gene cloning, blue-white screening, and plasmid stable amplification, etc.
When constructing plasmids, how should TOP10 and DH5α be selected?
In the majority of conventional plasmid amplification and blue-white screening experiments, the two can be used interchangeably.
Can it be repeatedly frozen and thawed?
It is strongly not recommended to use after repeated freeze-thaw cycles. The transformation activity of competent cells stored at -80℃ or transported with dry ice can be reduced by more than 50% after one freeze-thaw cycle.
