Cat.No.：D13041A
Price: ￥100
Specifications：
This product is a modified 10× concentrated DNA loading buffer. It contains two fluorescent dyes, bromophenol blue and xylene cyanol FF. It can meet the requirements for nucleic acid electrophoresis migration indication within the range of 300 bp to 4000 bp. After diluting to the working concentration of 1×, the solution's specific gravity remains at a relatively high level, making it easy to sink during loading. The color is highly distinguishable, facilitating real-time observation and judgment of the electrophoresis process.
The product formula has been optimized. Even after dilution to 1×, the density remains relatively high. During sample loading, it tends to sink. The indicator has good visibility. It contains two different indicators, which can indicate the electrophoresis process of nucleic acids ranging from 300bp to 4000bp.
| Cat. No. | Component | Specification | Quantity |
| B16041A | 10×DNA Loading Buffer | 1 mL | 5 |
2-8℃.
This product is suitable for nucleic acid agarose gel electrophoresis. It can indicate the electrophoresis process of nucleic acids with a minimum size of 200bp.
Can 10×DNA Loading Buffer be used for electrophoresis of RNA samples?
OK. However, before use, make sure that the 10×DNA Loading Buffer has not been contaminated by RNase. It is recommended to use RNase-free pipette tips for aspiration. If the sample is RNA, it is more advisable to use the dedicated RNA Loading Buffer to avoid the risk of RNA degradation.
Can the PCR products that have been added with 10× DNA Loading Buffer be directly subjected to the subsequent restriction enzyme reaction?
Sure. The dyes in the Loading Buffer do not affect the activity of the restriction endonucleases. The PCR products that have been added to the Loading Buffer can be directly subjected to the enzymatic digestion reaction.
What are the differences between 10×DNA Loading Buffer and 6×DNA Loading Buffer?
The core difference between the two lies in the concentration and the working dilution ratio. The 10× concentration is approximately 1.67 times that of the 6×. When using it, it needs to be diluted to the final concentration of 1× in different proportions. Additionally, some brands' 10× formulations contain SDS (dodecyl sulfate), while the 6× usually does not. The 10× Loading Buffer containing SDS can denature enzymes (such as Taq enzyme), and if used for PCR product electrophoresis, it may cause band dispersion. It is recommended to choose the appropriate product based on experimental requirements.
What size of DNA fragments do bromophenol blue and xylene cyanol FF indicate respectively?
In a 1% agarose gel, the migration rate of bromophenol blue is approximately equivalent to that of a 300 bp DNA fragment; the migration rate of xylene cyanol FF is approximately equivalent to that of a 4000 bp (4 kb) DNA fragment. These two dyes can assist in real-time monitoring of the electrophoresis process and can be used to stop the electrophoresis promptly when the target band reaches the appropriate position.
After adding the sample to the 10× DNA Loading Buffer, it floated out of the loading hole during the loading process. What is the reason for this?
Possible reasons include: Incorrect dilution ratio: The sample was not mixed with the 10× Loading Buffer in a 9:1 ratio, resulting in an insufficient final concentration of less than 1× and an insufficient density. Insufficient mixing: The sample and Loading Buffer were not thoroughly mixed, leading to uneven density. Improper sample addition operation: The pipette tip did not extend below the liquid surface of the addition hole, or the sample was ejected due to too fast dispensing speed. Residual ethanol in the sample: If it is a plasmid extraction product, there may be residual ethanol before elution. It is recommended to perform thorough centrifugation or vacuum removal.
After electrophoresis, the bands appear to be smeared or blurred. Is this related to the Loading Buffer?
It might be relevant. In the formulation of some 10× DNA Loading Buffer, SDS is included. SDS can denature proteins such as Taq enzyme, and when mixed with PCR products, it may cause the bands to become blurred. Additionally, band blurring can also be caused by the following reasons: DNA sample degradation (avoiding contamination by nucleases) Excessive loading volume Old electrophoresis buffer with reduced buffering capacity Excessive electrophoresis voltage (recommended not to exceed 20 V/cm) or excessive temperature
