Isothermal Amplification

Recombinase Polymerase Amplification (RPA) is a nucleic acid amplification technique that can be carried out at a constant temperature (37–42°C) without the need for a thermal cycler. This kit relies on recombinase (UvsX), single-stranded DNA-binding protein (GP32), and strand displacement DNA polymerase to achieve nucleic acid amplification under constant temperature conditions. Just by designing specific primers and adding the template, the amplification product can be obtained by incubating at 37°C for 10–40 minutes. The product can be detected through electrophoresis, real-time fluorescence (combined with Exo probe), or lateral flow test strips (LFS).

• Constant temperature reaction: The reaction can be carried out at 37–42°C, requiring only a simple heating block or water bath, without the need for a PCR machine;

• Ultra-fast amplification: The amplification can be completed in 10–40 minutes, much faster than conventional PCR;

• Convenient and rapid: It can be combined with colloidal gold test strips to achieve visual judgment by the naked eye, with extremely low dependence on equipment;

• Multiple compatibility: Multiple target detections can be achieved simultaneously by using different labeled primers.

Store at -20℃ and transport at ≤0℃

Isothermal library amplification (replacing PCR for NGS library construction), on-site rapid detection (for animal diseases, plant pathogens, food safety), bedside rapid diagnosis without PCR instrument, highly specific detection combined with CRISPR-Cas12/13 (SHERLOCK/DETECTR platforms).