Cat.No.：D16011A
Price: ￥
Specifications：
This product is specifically designed for efficient RNA quantitative detection, integrating reverse transcription and qPCR into a single reaction tube. The pre-mix contains heat-resistant reverse transcriptase, hot-start Taq DNA polymerase, RNase inhibitor, optimized buffer, dNTPs, and SYBR Green I or probe-based fluorescence system. As a single-tube integrated reagent, simply adding RNA template and primers/probes is sufficient to complete the entire process from RNA to quantitative results in one tube. This design not only significantly shortens the experimental period, but also minimizes sample cross-contamination and operational errors, ensuring the accuracy and stability of the experimental results.
One-tube operation: Reverse transcription and qPCR are completed in the same tube, reducing sample loss and cross-contamination caused by multiple pipetting operations;
High sensitivity: It can stably detect from pg-level total RNA or <100 copies of viral RNA;
Quick result: The total reaction time can be shortened to 40–60 minutes.
| Cat. No. | Component | Specification | Quantity |
| B23011A | One-Step RT-qPCR | 100 rxns | 1 |
Store at -20℃ and transport at ≤0℃
Real-time quantitative analysis of gene expression, quantitative analysis of trace precious RNA samples, rapid detection of viral RNA load, and rapid analysis of low-abundance gene expression.
How much RNA is required for the synthesis of the first chain of cDNA?
The amount of RNA template required for cDNA synthesis depends on individual needs. Generally, 1 μg of total RNA can be used for a routine 20-μL reverse transcription reaction (with no more than 2 μg of RNA in the 10 μL reaction system).
Compared to traditional RT-qPCR, what are the advantages and disadvantages of the one-tube pre-mix solution?
A one-tube continuous closed-loop operation involves fewer pipetting steps, has an extremely low risk of cross-contamination, and is time-saving and labor-saving. It is highly suitable for clinical diagnosis and high-throughput large-scale sample screening. However, due to the shared buffer system for reverse transcription and qPCR, the reaction conditions often compromise between the two, thus making it impossible to separate the two-step reactions.
