Cat.No.：D15021A
Price: ￥
Specifications：
This product is specifically developed for qPCR using hydrolysis probes such as TaqMan® and molecular beacons. It is a highly integrated 2× premix solution. The kit contains a thermostable Taq DNA polymerase with 5'→3' exonuclease activity, optimized buffer, dNTPs, and Mg²⁺. The product adopts a dye-free formula and releases fluorescence signals through the specific binding of the hydrolysis probe and enzymatic cleavage. Compared to traditional dye-based methods, this premix solution demonstrates higher detection specificity and signal-to-noise ratio, and can precisely meet various high-demand quantitative analysis requirements.
High specificity of the probe method: It generates a signal only when the probe perfectly matches the target, eliminating the interference of primer dimerization;
Multiple detection capability: Supports simultaneous detection with multi-color probes such as FAM/VIC/ROX/CY5;
Low background and high signal-to-noise ratio: The optimized buffer system ensures low fluorescence background and high signal intensity;
Fast activation: The heat-labeled antibody is fully activated within 30 seconds at 95°C, supporting rapid qPCR procedures.
| Cat. No. | Component | Specification | Quantity |
| D21011A | Probe qPCR Mix (2×) | 1 mL | 1 |
Store at -20℃ and transport at ≤0℃
Pathogenic microorganism absolute quantification, quantitative detection of transgenic organisms, absolute quantification of copy number variation, SNP genotyping, multiplex qPCR.
What are the advantages of using probe-based fluorescent dye quantification compared to other methods?
Compared with the dye method, the probe method has extremely high specificity. The generation of fluorescence signals not only requires the primers to bind to the template, but also requires the probe to precisely bind to the target sequence between the upstream and downstream primers. This "double verification" mechanism significantly reduces the interference of non-specific amplification and usually does not require melting curve analysis, resulting in more accurate and reliable results.
The melting curve has multiple peaks. How should we deal with this?
When multiple peaks appear in the melting curve, it indicates the presence of non-specific amplification or primer dimers. The main reason is poor primer design (such as the presence of dimers, hairpin structures, or a significant difference in Tm values between upstream and downstream primers). It is recommended to redesign the primers, appropriately reduce the primer concentration, or increase the annealing temperature to enhance specificity.
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