Products

Nucleic Acid Extraction

Universal RNA Extraction Kit

Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit
Universal RNA Extraction Kit

Cat.No.:D24021A

Price:

Specifications:

  • 50 rxns
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Product Description

The Universal RNA Extraction Kit is a reagent set designed to extract total RNA from animal cells or tissues, plant samples, and microorganisms. This product is an improved version based on the Trizol method, integrating the isothiocyanate guanidine/phenol/chloroform method to extract total RNA from the samples. It efficiently releases nucleic acids while inactivating RNase, ensuring the quality of the extracted RNA. The product can be used in experiments such as cDNA cloning, RT-PCR, RT-qPCR, mRNA purification, Northern-blotting, sequencing, etc.

Product Advantages

  • Operation is convenient: The steps are clear and the operation is simple.

  • Product quality is high: The RNA purity is high and the integrity is good. It removes protein and other impurities to the greatest extent, with OD260/OD280 > 1.9.

  • The application range is wide: It is suitable for animal cells or tissues, plants, microorganisms and other samples, and can obtain total RNA including all miRNAs.

Product Specifications

Cat. No.ComponentSpecificationQuantity
B53011ALysis Buffer30 mL1
B53021AWash Buffer I8 mL1
B53031AWash Buffer II8 mL1
B53041ARNA Resuspension Buffer15 mL1
B53051ASpin Columns and Collection Tubes50 sets1

Storage Conditions

The lysis solution should be stored at 4℃, while the other components should be kept at room temperature. The product should be transported at ≤ 0℃.

Product Applications

This product is suitable for extracting total RNA from animal cells or tissues obtained through cultivation, as well as from plants and microorganisms.

The concentration of the extracted RNA product is low. How to solve this problem?

During the operation, please ensure that the bacterial cells are completely lysed. For certain yeasts and bacteria, if the lysis is not thorough enough, a homogenizer can be used for homogenization to ensure complete lysis.

The extracted DNA product has an A260/280 ratio of less than 1.8.

An A260/A280 ratio less than 1.8 usually indicates the presence of DNA or protein contamination. Please ensure that the lysis buffer (containing proteinase K) in the kit is properly stored at -20℃.

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