Cat.No.：D12011A
Price: ￥100
Specifications：
The PCR enhancer is a broad-spectrum PCR reaction additive designed to significantly enhance the amplification efficiency and specificity of difficult templates. Its core mechanism lies in optimizing the microenvironment of the DNA polymerase, stabilizing the primer-template complex, and effectively lowering the denaturation temperature of the template secondary structure. GC Enhancer has excellent compatibility and fully supports various DNA polymerases such as Taq, Pfu, high fidelity, and thermal start. In practical applications, users can add it as needed to any PCR reaction system according to the amplification requirements, effectively overcoming the amplification obstacles caused by high GC or complex structure templates.
Broad-spectrum compatibility: applicable to various DNA polymerases such as Taq, high fidelity, and hot start;
Ready-to-use concentrated solution: simply add by volume;
Significantly improves success rate: the success rate of difficult templates that failed in conventional PCR is increased by more than 80%.
| Cat. No. | Component | Specification | Quantity |
| B12011A | PCR Enhancer | 1 mL | 1 |
Store at -20℃ and transport at ≤0℃
Auxiliary amplification of high GC-content templates, enhancement of amplification for crude extracted samples (soil, feces, blood) containing PCR inhibitors, improvement of the yield and specificity of long fragment PCR (>5 kb), balancing the amplification efficiency of each target in multiplex PCR, enhancement of the sensitivity for detecting low-copy templates, and optimization of difficult PCR systems for crude extracted nucleic acids containing impurities.
Under what conditions is the PCR Enhancer usually used?
PCR Enhancer can effectively enhance the amplification efficiency of fragments with a length of ≥ 4 kbp and high GC templates (with a GC content > 70%). It can also effectively reduce the background of amplification products and inhibit the generation of non-specific bands.
Will adding PCR Enhancer to a PCR system with normal amplification effect have any impact on the amplification process?
This may affect the stability of the primer-template pairing, so it is not necessary to add it unless it is absolutely necessary.
