Direct PCR

Plant Tissue Direct Amplification PCR Master Mix (2×) This product is a highly efficient direct amplification reagent specially developed to overcome the PCR inhibition barriers in plant samples. It is designed to address the complex inhibitors such as polysaccharides and polyphenols commonly found in plant tissues. Relying on inhibitor-tolerant DNA polymerase and exclusive enhanced buffer system, this product demonstrates outstanding anti-inhibition performance. During the experiment, simply crushing the tissues like leaves, seeds or root tips is sufficient to directly use them as templates for amplification, eliminating the need for conventional DNA extraction and purification steps. This reagent ensures accurate and stable amplification results while significantly simplifying the sample pre-treatment process, providing a reliable and efficient solution for plant molecular biology research.

• Plant inhibitor tolerance: Exhibits outstanding tolerance to plant-specific inhibitors such as polyphenols, polysaccharides, tannins, etc.;

• One-step lysis direct amplification: After punching holes or grinding the leaves, they are directly added to the reaction system;

• High-throughput compatibility: Compatible with 96/384 well plate automated operations;

• High success rate: Verified effective for major crops such as rice, wheat, corn, soybeans, and Arabidopsis thaliana.

Store at -20℃ and transport at ≤0℃

Positive identification of transgenic plants, screening of crop germplasm resources, detection of plant pathogens (fungi, bacteria, viruses), genotyping of plant leaves/seeds, identification of callus tissues, etc.