Cat.No.：D18011A
Price: ￥
Specifications：
High-purity dNTP Mix is a core basic raw material specially developed for nucleic acid amplification and molecular biology experiments. It is widely applicable to key applications such as PCR, RT-PCR, qPCR, DNA sequencing and molecular cloning. This product adopts an optimized aqueous solution system and contains equimolar concentrations of deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dTTP). All components are strictly quality-controlled by HPLC, with a purity of ≥ 99%, and ensure no contamination from DNase, RNase and human genomic DNA. This product exhibits excellent compatibility and can work in synergy with all standard DNA polymerases, providing a stable and reliable material foundation for various high-demand molecular biology experiments.
Ultra-high purity: HPLC purity ≥ 99%, no cross-contamination of modified nucleotides;
Batch stability: Strict quality control ensures consistency in concentration and purity between batches;
Ready-to-use concentration: Available in 2.5 mM each (for routine PCR) and 10 mM each (for experiments requiring high concentration);
No nuclease contamination: Verified by DNase, RNase and protease activity tests.
| Cat. No. | Component | Specification | Quantity |
| B25011A | dNTP Mix, 10mM each | 0.5 mL | 1 |
| B25012A | dNTP Mix, 10mM each | 1 mL | 1 |
Store at -20℃ and transport at ≤0℃
Various PCR reactions (conventional PCR, high-fidelity PCR, thermal-start PCR), cDNA reverse transcription synthesis, DNA sequencing, DNA labeling experiments, and the preparation of various in vitro enzymatic nucleic acid synthesis systems.
How should it be diluted when using it? What should be noted?
When dilution is required, it is recommended to use sterile distilled water or PCR-grade water for dilution. Avoid using solutions containing DNase/RNase.
Adding dNTP mix during PCR amplification results in the formation of non-specific bands.
High concentrations of dNTPs may increase non-specific amplification. It is recommended to use 0.2 mM (for each dNTP). For long fragment PCR or qPCR, high-purity dNTPs (such as those with HPLC purity > 99%) are recommended.
