Cat.No.:D02031A
Price: ¥12
Specifications:
It contains molecularly modified Taq DNA polymerase, dNTPs and optimized reaction buffer. During amplification, only template, primers and water need to be added. The core Taq DNA polymerase, after molecular modification, has extremely high amplification efficiency and wide template adaptability. It possesses 5′→3′ exonuclease activity, and the 3′ end of the amplification product does not require template addition of an A tail. The performance advantages of this enzyme are prominent: the amplification speed is fast (≤ 15 seconds/1 kbp), it has efficient amplification ability for complex templates such as plant genomic DNA and blood, and at the same time, it has high sensitivity and good tolerance to amplification inhibitors. The unique stabilizer added in the premix can ensure that the amplification performance of the product remains stable without significant fluctuations after 25 repeated freeze-thaw cycles, and the stability of use is excellent.
Fast and efficient: Short fragments can reduce extension time, and the extension speed is 2-3 times higher than that of the wild-type Taq;
Cost optimization: Excellent cost performance, achieving a balance between rapid performance and economy;
High yield: The natural high amplification efficiency of Taq enzyme ensures the yield of the product;
Ready-to-use premix: Reduces the number of operation steps, replaces the traditional self-preparation system of Taq + buffer, and improves the reproducibility of the experiment.
| Cat. No. | Component | Specification | Quantity |
| B01031A | Taq PCR Master Mix (2×) | 1 mL | 1 |
| B01031A | Taq PCR Master Mix (2×) | 1 mL | 5 |
Store at -20℃ and avoid repeated freezing and thawing as much as possible.
Gene amplification, DNA fragment splicing, complete genome synthesis, etc.
What are the differences between the rapid Taq enzyme and the ordinary Taq enzyme?
Compared to the ordinary Taq enzyme which takes 1 minute per kbp, the rapid Taq enzyme has optimized the sequence to increase the amplification speed to 15 seconds per kbp, saving a significant amount of time. At the same time, it provides accurate quantification and reliability on the qPCR platform in a shorter time.
Can Fast Taq be used to amplify long fragments?
Sure, using the plasmid as the template, we successfully amplified a distinct 8kbp target band with Taq enzyme. However, if you aim for extremely high amplification yield, it is not recommended to amplify DNA fragments larger than 2kbp.
