Hot Start PCR

This product employs chemically modified heat-initiated Taq DNA polymerase. The activity is regulated through antibody blocking or chemical modification techniques: at room temperature, the polymerase activity is completely inhibited, and it is only activated specifically during the initial denaturation step at ≥95°C. This design can completely avoid the problems of non-specific primer binding and primer dimer formation that may occur during the low-temperature preparation of the reaction system. It significantly improves the specificity and sensitivity of the PCR reaction and is the preferred reagent for complex template amplification, construction of multiplex PCR systems, and detection of low-copy templates.

• Zero room-temperature activity: Antibodies/chemical modifications ensure no leakage of polymerase activity during the configuration process;

• Extremely high specificity: Significantly reduces non-specific amplification and primer dimerization;

• High sensitivity: Capable of detecting single-copy targets;

• Wide dynamic range: Suitable for template input volumes ranging from pg level to μg level.

The heat activation enzyme needs to activate its activity at high temperatures. It is recommended to set the pre-denaturation time at 3-5 minutes when using it. An excessively long pre-denaturation process will result in the enzyme losing some of its activity, while a too short time will prevent the enzyme from being fully activated.

Store at -20℃ and transport at ≤0℃

Low-copy number template amplification, multiple nested PCR, micro-sized pathogen nucleic acid amplification, methylation-specific PCR, amplification experiments prone to primer dimer formation.